Animal models in dengue

Emily Plummer; Sujan Shresta

doi:10.1007/978-1-4939-0348-1_23

Animal models in dengue

Methods Mol Biol. 2014:1138:377-90. doi: 10.1007/978-1-4939-0348-1_23.

Authors

Emily Plummer¹, Sujan Shresta

Affiliation

¹ Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA, 92037, USA.

PMID: 24696349
DOI: 10.1007/978-1-4939-0348-1_23

Abstract

Validation of a mouse model of dengue virus (DENV) infection relies on verification of viremia and productive replication in mouse tissues following infection. Here, we describe a quantitative assay for determining viral RNA levels in mouse serum and tissues. For the purpose of confirming DENV replication, we outline a fluorescence immunohistochemistry (FIHC) protocol for staining a nonstructural protein of DENV.

MeSH terms

Animals
Dengue / virology*
Dengue Virus / genetics
Disease Models, Animal*
Fluorescence
Immunohistochemistry
Mice
RNA / isolation & purification
RNA Helicases / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Serine Endopeptidases / metabolism
Spleen / virology
Viral Nonstructural Proteins / metabolism

Substances

NS3 protein, flavivirus
Viral Nonstructural Proteins
RNA
Serine Endopeptidases
RNA Helicases