Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

J Clin Microbiol. 2014 Jun;52(6):2196-8. doi: 10.1128/JCM.00039-14. Epub 2014 Apr 2.

Abstract

Saprochaete clavata and Magnusiomyces capitatus are human pathogens that are frequently mistaken for each other due to their similar phenotypes and erroneous or limited databases. Based on internal transcribed spacer (ITS) sequences, we propose species-specific carbon assimilation patterns and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) fingerprints that enable the identification of S. clavata, M. capitatus, and Galactomyces candidus to the species level.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon / metabolism
  • Cluster Analysis
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Diagnostic Tests, Routine / methods*
  • Humans
  • Molecular Sequence Data
  • Mycological Typing Techniques
  • Mycoses / diagnosis*
  • Mycoses / microbiology*
  • Phylogeny
  • Saccharomycetales / chemistry
  • Saccharomycetales / classification*
  • Saccharomycetales / genetics
  • Saccharomycetales / isolation & purification*
  • Sequence Analysis, DNA*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • DNA, Fungal
  • DNA, Ribosomal Spacer
  • Carbon

Associated data

  • GENBANK/KF984489
  • GENBANK/KF984490
  • GENBANK/KF984491