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Anal Biochem. 1988 Nov 15;175(1):298-9.

Alcian blue staining of proteoglycans in polyacrylamide gels using the "critical electrolyte concentration" approach.

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1
Open University, Oxford Research Unit, United Kingdom.

Abstract

A method is described here for Alcian blue staining of proteoglycans in polyacrylamide gels; this is illustrated using extracts obtained from bovine corneal stroma. Other available methods for visualization of proteoglycans can produce nonspecific staining and frequently a high background in the gel; with a "critical electrolyte concentration" approach, specific staining against a clear background can be obtained.

PMID:
2469353
[Indexed for MEDLINE]
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