Format

Send to

Choose Destination
J Exp Bot. 2014 Jul;65(13):3715-24. doi: 10.1093/jxb/eru130. Epub 2014 Apr 1.

Photosynthetic flexibility in maize exposed to salinity and shade.

Author information

1
Hawkesbury Institute for the Environment, University of Western Sydney, Richmond, Locked bag 1797, Penrith NSW 2751, Australia.
2
Hawkesbury Institute for the Environment, University of Western Sydney, Richmond, Locked bag 1797, Penrith NSW 2751, Australia o.ghannoum@uws.edu.au.

Abstract

C4 photosynthesis involves a close collaboration of the C3 and C4 metabolic cycles across the mesophyll and bundle-sheath cells. This study investigated the coordination of C4 photosynthesis in maize plants subjected to two salinity (50 and 100mM NaCl) treatments and one shade (20% of full sunlight) treatment. Photosynthetic efficiency was probed by combining leaf gas-exchange measurements with carbon isotope discrimination and assaying the key carboxylases [ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC)] and decarboxylases [nicotinamide adenine dinucleotide phosphate malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PEP-CK)] operating in maize leaves. Generally, salinity inhibited plant growth and photosynthesis to a lesser extent than shade. Salinity reduced photosynthesis primarily by reducing stomatal conductance and secondarily by equally reducing Rubisco and PEPC activities; the decarboxylases were inhibited more than the carboxylases. Salinity increased photosynthetic carbon isotope discrimination (Δp) and reduced leaf dry-matter carbon isotope composition ((13)δ) due to changes in p i/p a (intercellular to ambient CO2 partial pressure), while CO2 leakiness out of the bundle sheath (ϕ) was similar to that in control plants. Acclimation to shade was underpinned by a greater downregulation of PEPC relative to Rubisco activity, and a lesser inhibition of NADP-ME (primary decarboxylase) relative to PEP-CK (secondary decarboxylase). Shade reduced Δp and ɸ without significantly affecting leaf (13)δ or p i/p a relative to control plants. Accordingly, shade perturbed the balance between the C3 and C4 cycles during photosynthesis in maize, and demonstrated the flexible partitioning of C4 acid decarboxylation activity between NADP-ME and PEP-CK in response to the environment. This study highlights the need to improve our understanding of the links between leaf (13)δ and photosynthetic Δp, and the role of the secondary decarboxylase PEP-CK in NADP-ME plants such as maize.

KEYWORDS:

C4 photosynthesis; NADP-ME; PEP-CK; PEPC; Rubisco.; carbon isotope discrimination; leakiness

PMID:
24692650
PMCID:
PMC4085963
DOI:
10.1093/jxb/eru130
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center