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Virus Genes. 2014 Jun;48(3):550-2. doi: 10.1007/s11262-014-1057-9. Epub 2014 Apr 2.

Detection of enterovirus genome sequence from diarrheal feces of goat.

Author information

1
Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo, 183-8509, Japan.

Abstract

Goat diarrheal feces were subjected to metagenome analysis by the next-generation sequencing. Nucleotide sequences with homology to enteroviruses were obtained. Primers for RT-PCR were designed based on the nucleotide sequence of these sequences at the 5'-untranslated region, and we determined 563 bp nucleotide sequences that showed homology to bovine-like and ovine enteroviruses (77-87 %). We named the virus detected in this study goat enterovirus G1 (GEV-G1). In the phylogenetic analysis, GEV-G1 belonged to a cluster containing ovine enteroviruses. To our knowledge, this is the first report on nucleotide sequences of an enterovirus infecting Japanese goats.

PMID:
24691818
DOI:
10.1007/s11262-014-1057-9
[Indexed for MEDLINE]

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