Format

Send to

Choose Destination
Biomaterials. 2014 Jun;35(19):5098-109. doi: 10.1016/j.biomaterials.2014.03.011. Epub 2014 Mar 29.

Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate.

Author information

1
Department of Chemical Engineering, University of South Carolina, SC 29208, USA.
2
Graduate Aeronautics Laboratories, California Institute of Technology, CA 91125, USA.
3
Department of Biomedical Engineering, University of South Carolina, SC 29208, USA.
4
Department of Chemistry and Biochemistry, University of South Carolina, SC 29208, USA.
5
Department of Chemical Engineering, University of South Carolina, SC 29208, USA; Department of Biomedical Engineering, University of South Carolina, SC 29208, USA; Department of Orthopaedic Surgery, University of South Carolina, SC 29208, USA. Electronic address: jabbarza@cec.sc.edu.

Abstract

We investigated the biological response of human pluripotent stem cells (hPSCs) cultured on a carbon nanotube (CNT) array-based substrate with the long term goal to direct hPSC germ layer specification for a wide variety of tissue engineering applications. CNT arrays were fabricated using a chemical vapor deposition system allowing for control over surface roughness and mechanical stiffness. Our results demonstrated that hPSCs readily attach to hydrophilized and extracellular matrix coated CNT arrays. hPSCs cultured as colonies in conditions supporting self-renewal demonstrated the morphology and marker expression of undifferentiated hPSCs. Conditions inducing spontaneous differentiation lead to hPSC commitment to all three embryonic germ layers as assessed by immunostaining and RT-PCR analysis. Strikingly, the physical characteristics of CNT arrays favored mesodermal specification of hPSCs. This is contradictory to the behavior of hPSCs on traditional tissue culture plastic which promotes the development of ectoderm. Altogether, these results demonstrate the potential of CNT arrays to be used in the generation of new platforms that allow for precise control of hPSC differentiation by tuning the characteristics of their physical microenvironment.

KEYWORDS:

Cell adhesion; Cytoskeleton; Differentiation; Human pluripotent stem cells; Multi-walled carbon nanotubes; Surface roughness

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center