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Biochem Biophys Res Commun. 2014 Apr 25;447(1):19-25. doi: 10.1016/j.bbrc.2014.03.108. Epub 2014 Mar 29.

Monitoring of the serum proteome in Kawasaki disease patients before and after immunoglobulin therapy.

Author information

1
Guangzhou Women and Children's Medical Center, Guangzhou 510120, Guangdong, China.
2
Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China.
3
Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China.
4
Guangdong Institute of Microbiology/State Key Laboratory of Applied Microbiology, Ministry-Guangdong Province Jointly Breeding Base, Guangzhou 510070, Guangdong, China.
5
Guangzhou Women and Children's Medical Center, Guangzhou 510120, Guangdong, China. Electronic address: de_dengli@163.com.
6
Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China. Electronic address: zhanggong@jnu.edu.cn.

Abstract

Kawasaki disease (KD) is a systemic vasculitis that mainly affects children younger than 5 years. The causal pathogen is unknown, therefore specific diagnostic biomarkers and therapy are unavailable. High-dose intravenous immunoglobulin (IVIG) is considered as the most effective therapy to reduce the prevalence of coronary artery lesion (CAL) in KD; however, it has side effects. This study aimed to (1) determine whether IVIG therapy is effective at the molecular level; (2) provide the first serum proteomic profile of KD under IVIG therapy; and (3) screen for monitoring biomarker candidates. We extracted serum proteins from samples of healthy individuals and from KD patients before and after IVIG therapy, and employed two-dimensional electrophoresis and MALDI-TOF/TOF mass spectrometry to identify differentially expressed proteins. The identifications were validated by Western blotting. We identified 29 differentially expressed proteins in KD patients and found that IVIG therapy restored most of these proteins to near-normal levels. Tracing the protein levels of single patients before and after IVIG therapy showed that the proteins, especially Transthyretin (TTR), are potential markers for therapeutic monitoring. Functional analyses of these proteins by PANTHER and String suggested that the key influence of KD lay in the immune system, which was targeted by IVIG.

KEYWORDS:

Differentially expressed proteins; Intravenous immunoglobulin; Kawasaki disease; Proteome

PMID:
24690176
DOI:
10.1016/j.bbrc.2014.03.108
[Indexed for MEDLINE]
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