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J Cardiovasc Pharmacol. 1989 Jan;13(1):168-72.

Effect of albumin on the electrophysiologic stability of isolated perfused rabbit hearts.

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Cardiology Division, Falk Cardiovascular Research Center, Stanford University Medical Center, California 94305.


The electrophysiologic stability of isolated perfused rabbit hearts was evaluated over a period of 5 h. Hearts perfused with protein-free buffer deteriorated over time, with significant shortening of the ventricular effective refractory period (ERP) and development of ventricular fibrillation. When serum albumin (6.01 x 10(-4) mM) was added to the perfusate, hearts were more stable, the ventricular ERPs remained relatively constant throughout the 5-h perfusion period, and hearts did not fibrillate. When a fatty acid-free protein source was used, the hearts demonstrated similar stability to those perfused with protein containing fatty acids. Despite marked changes in the refractory periods of hearts perfused with protein-free buffer, the QRS interval did not change over time, indicating that this is a very insensitive parameter for monitoring the electrophysiologic stability of isolated perfused hearts. In studies utilizing isolated hearts to evaluate antiarrhythmic drug effects, it is preferable to use a protein-containing buffer.

[Indexed for MEDLINE]

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