Format

Send to

Choose Destination
See comment in PubMed Commons below
Biomed Res Int. 2014;2014:760303. doi: 10.1155/2014/760303. Epub 2014 Feb 10.

The low chamber pancreatic cancer cells had stem-like characteristics in modified transwell system: is it a novel method to identify and enrich cancer stem-like cells?

Author information

1
The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
2
Department of Histology and Embryology, Center of Clinical Medicine and Laboratory, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
3
Department of Immunology, Center of Clinical Medicine and Laboratory, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.

Abstract

Cancer stem cells (CSCs) or cancer-initiating cells (CICs) play an important role in tumor initiation, progression, metastasis, chemoresistance, and recurrence. It is important to construct an effective method to identify and isolate CSCs for biotherapy of cancer. During the past years, many researchers had paid more attention to it; however, this method was still on seeking. Therefore, compared to the former methods that were used to isolate the cancer stem cell, in the present study, we tried to use modified transwell system to isolate and enrich CSCs from human pancreatic cancer cell lines (Panc-1). Our results clearly showed that the lower chamber cells in modified transwell system were easily forming spheres; furthermore, these spheres expressed high levels of stem cell markers (CD133/CD44/CD24/Oct-4/ESA) and exhibited chemoresistance, underwent epithelial-to-mesenchymal transition (EMT), and possessed the properties of self-renewal in vitro and tumorigenicity in vivo. Therefore, we speculated that modified transwell assay system, as a rapid and effective method, can be used to isolate and enrich CSCs.

PMID:
24689055
PMCID:
PMC3934619
DOI:
10.1155/2014/760303
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Hindawi Publishing Corporation Icon for PubMed Central
    Loading ...
    Support Center