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Antimicrob Agents Chemother. 2014 Jun;58(6):3538-40. doi: 10.1128/AAC.02793-13. Epub 2014 Mar 31.

Biochemical analysis of metallo-β-lactamase NDM-3 from a multidrug-resistant Escherichia coli strain isolated in Japan.

Author information

1
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo, Japan.
2
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo, Japan tkirikae@ri.imcj.go.jp.

Abstract

New Delhi metallo-β-lactamase-3 (NDM-3) was identified in a multidrug-resistant Escherichia coli isolate, NCGM77, obtained from the feces of a patient in Japan. The enzymatic activities of NDM-3 against β-lactams were similar to those of NDM-1, although NDM-3 showed slightly lower kcat/Km ratios for all the β-lactams tested except for doripenem. The genetic context for blaNDM-3 was tnpA-blaNDM-3-bleMBL-trpF-dsbC-tnpA-sulI-qacEdeltaI-aadA2-dfrA1, which was present on an approximately 250-kb plasmid.

PMID:
24687501
PMCID:
PMC4068495
DOI:
10.1128/AAC.02793-13
[Indexed for MEDLINE]
Free PMC Article

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