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Chem Commun (Camb). 2014 May 18;50(38):4891-4. doi: 10.1039/c4cc01528b.

A diazirine-based photoaffinity probe for facile and efficient aptamer-protein covalent conjugation.

Author information

1
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, P. R. China. cyyang@xmu.edu.cn zhuzhi@xmu.edu.cn.

Abstract

A photo-reactive functional labelling reagent, diazirine phosphoramidite, was designed and synthesized for easy and flexible site-specific labelling of oligonucleotides with the diazirine moiety. The new reagent allows facile photo-crosslinking of oligonucleotide with its interacting partner for a variety of applications, including tertiary structure determination, molecular interaction study and biomarker discovery.

PMID:
24686985
DOI:
10.1039/c4cc01528b
[Indexed for MEDLINE]
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