Format

Send to

Choose Destination
J Immunol Methods. 2014 May;407:40-7. doi: 10.1016/j.jim.2014.03.018. Epub 2014 Mar 28.

Reproducibility studies for experimental epitope detection in macrophages (EDIM).

Author information

1
Department of General Surgery, Atrium Medical Center Parkstad, Henri Dunantstraat 5, 6419 PC Heerlen, The Netherlands; Department of General Surgery, Maastricht University Medical Center, P. Debyelaan 25, 6229 HX Maastricht, The Netherlands; GROW-School for Oncology and Developmental Biology, Maastricht University Medical Center, P. Debyelaan 25, 6229 HX Maastricht, The Netherlands. Electronic address: dennisjapink@gmail.com.
2
Department of Clinical Pathology, Atrium Medical Center Parkstad, Henri Dunantstraat 5, 6419 PC Heerlen, The Netherlands. Electronic address: m.nap@atriummc.nl.
3
Department of General Surgery, Atrium Medical Center Parkstad, Henri Dunantstraat 5, 6419 PC Heerlen, The Netherlands. Electronic address: m.sosef@atriummc.nl.
4
Department of Epidemiology, Maastricht University Medical Center, P. Debyelaan 25, 6229 HX Maastricht, The Netherlands. Electronic address: patty.nelemans@maastrichtuniversity.nl.
5
TAVARLIN AG, Reißstraße 1a, 64319 Pfungstadt, Germany. Electronic address: j.coy@tavarlin.de.
6
Department of General Surgery, Maastricht University Medical Center, P. Debyelaan 25, 6229 HX Maastricht, The Netherlands. Electronic address: g.beets@maastrichtuniversity.nl.
7
Department of General Surgery, Maastricht University Medical Center, P. Debyelaan 25, 6229 HX Maastricht, The Netherlands. Electronic address: mf.vonmeyenfeldt@maastrichtuniversity.nl.
8
Department of Clinical Chemistry and Hematology, Atrium Medical Center Parkstad, Henri Dunantstraat 5, 6419 PC Heerlen, The Netherlands. Electronic address: m.leers@atriummc.nl.

Abstract

INTRODUCTION:

We have recently described epitope detection in macrophages (EDIM) by flow cytometry. This is a promising tool for the diagnosis and follow-up of malignancies. However, biological and technical validation is warranted before clinical applicability can be explored.

METHODS:

The pre-analytic and analytic phases were investigated. Five different aspects were assessed: blood sample stability, intra-individual variability in healthy persons, intra-assay variation, inter-assay variation and assay transferability. The post-analytic phase was already partly standardized and described in an earlier study.

RESULTS:

The outcomes in the pre-analytic phase showed that samples are stable for 24h after venipuncture. Biological variation over time was similar to that of serum tumor marker assays; each patient has a baseline value. Intra-assay variation showed good reproducibility, while inter-assay variation showed reproducibility similar to that of to established serum tumor marker assays. Furthermore, the assay showed excellent transferability between analyzers.

CONCLUSION:

Under optimal analytic conditions the EDIM method is technically stable, reproducible and transferable. Biological variation over time needs further assessment in future work.

KEYWORDS:

Analytic; Colorectal Cancer; Epitope detection in macrophages; Flowcytometry; Pre-analytic; Reproducibility

PMID:
24685835
DOI:
10.1016/j.jim.2014.03.018
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center