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J Microbiol Methods. 2014 Jun;101:24-7. doi: 10.1016/j.mimet.2014.03.008. Epub 2014 Mar 28.

Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing.

Author information

1
INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France.
2
Division of Evolution, Ecology & Genetics, Research School of Biology, The Australian National University, Canberra ACT 0200, Australia.
3
INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Nord, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France.
4
INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France. Electronic address: erick.denamur@inserm.fr.

Abstract

We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.

KEYWORDS:

Allele-specific PCR; B2 phylogroup; B2 sub-group; Escherichia coli; Sequence type complex

PMID:
24685601
DOI:
10.1016/j.mimet.2014.03.008
[Indexed for MEDLINE]

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