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Mol Cell. 2014 Apr 10;54(1):147-155. doi: 10.1016/j.molcel.2014.02.024. Epub 2014 Mar 27.

An mRNA-derived noncoding RNA targets and regulates the ribosome.

Author information

1
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland.
2
Division of Genomics and RNomics, Medical University Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria; Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego Street 12/14, 61-704 Poznan, Poland.
3
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland.
4
Division of Genomics and RNomics, Medical University Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria; Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland.
5
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland; Division of Genomics and RNomics, Medical University Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria. Electronic address: norbert.polacek@dcb.unibe.ch.

Abstract

The structural and functional repertoire of small non-protein-coding RNAs (ncRNAs) is central for establishing gene regulation networks in cells and organisms. Here, we show that an mRNA-derived 18-nucleotide-long ncRNA is capable of downregulating translation in Saccharomyces cerevisiae by targeting the ribosome. This 18-mer ncRNA binds to polysomes upon salt stress and is crucial for efficient growth under hyperosmotic conditions. Although the 18-mer RNA originates from the TRM10 locus, which encodes a tRNA methyltransferase, genetic analyses revealed the 18-mer RNA nucleotide sequence, rather than the mRNA-encoded enzyme, as the translation regulator. Our data reveal the ribosome as a target for a small regulatory ncRNA and demonstrate the existence of a yet unkown mechanism of translation regulation. Ribosome-targeted small ncRNAs are found in all domains of life and represent a prevalent but so far largely unexplored class of regulatory molecules.

PMID:
24685157
PMCID:
PMC3988847
DOI:
10.1016/j.molcel.2014.02.024
[Indexed for MEDLINE]
Free PMC Article

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