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Int J Cardiol. 2014 May 15;173(3):410-23. doi: 10.1016/j.ijcard.2014.03.015. Epub 2014 Mar 14.

Direct implantation versus platelet-rich fibrin-embedded adipose-derived mesenchymal stem cells in treating rat acute myocardial infarction.

Author information

1
Department of Emergency Medicine, E-DA Hospital, I-Shou University, Kaohsiung, Taiwan.
2
Division of Cardiology, Department of Internal Medicine, Taiwan.
3
Center for Translational Research in Biomedical Sciences, Taiwan.
4
Basic Science, Nursing Department, Meiho Institute of Technology, Pingtung, Taiwan.
5
Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
6
Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan.
7
Division of Cardiology, Department of Internal Medicine, Taiwan; Center for Translational Research in Biomedical Sciences, Taiwan. Electronic address: han.gung@msa.hinet.net.

Abstract

BACKGROUND:

This study tested whether adipose-derived mesenchymal stem cells (ADMSC) embedded in platelet-rich fibrin (PRF) scaffold is superior to direct ADMSC implantation in improving left ventricular (LV) performance and reducing LV remodeling in a rat acute myocardial infarction (AMI) model of left anterior descending coronary artery (LAD) ligation.

METHODS:

Twenty-eight male adult Sprague Dawley rats equally divided into group 1 [sham control], group 2 (AMI only), group 3 (AMI+direct ADMSC implantation), and group 4 (AMI+PRF-embedded autologous ADMSC) were sacrificed on day 42 after AMI.

RESULTS:

LV systolic and diastolic dimensions and volumes, and infarct/fibrotic areas were highest in group 2, lowest in group 1 and significantly higher in group 3 than in group 4, whereas LV performance and LV fractional shortening exhibited a reversed pattern (p<0.005). Protein expressions of inflammation (oxidative stress, IL-1β, MMP-9), apoptosis (mitochondrial Bax, cleaved PARP), fibrosis (Smad3, TGF-β), and pressure-overload biomarkers (BNP, MHC-β) displayed a pattern similar to that of LV dimensions, whereas anti-inflammatory (IL-10), anti-apoptotic (Bcl-2), and anti-fibrotic (Smad1/5, BMP-2) indices showed a pattern similar to that of LV performance among the four groups (all p<0.05). Angiogenesis biomarkers at protein (CXCR4, SDF-1α, VEGF), cellular (CD31+, CXCR4+, SDF-1α+), and immunohistochemical (small vessels) levels, and cardiac stem cell markers (C-kit+, Sca-1+) in infarct myocardium were highest in group 4, lowest in group 1, and significantly higher in group 3 than in group 2 (all p<0.005).

CONCLUSION:

PRF-embedded ADMSC is superior to direct ADMSC implantation in preserving LV function and attenuating LV remodeling.

KEYWORDS:

Acute myocardial infarction; Adipose-derived mesenchymal stem cell; Angiogenesis; Left ventricular remodeling; Platelet-rich fibrin scaffold

PMID:
24685001
DOI:
10.1016/j.ijcard.2014.03.015
[Indexed for MEDLINE]

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