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Dev Cell. 2014 Apr 14;29(1):112-27. doi: 10.1016/j.devcel.2014.02.018. Epub 2014 Mar 27.

Functional genomic analysis of the periodic transcriptome in the developing Drosophila wing.

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Stowers Institute for Medical Research, 1000 East 50(th) Street, Kansas City, MO 64110, USA.
Stowers Institute for Medical Research, 1000 East 50(th) Street, Kansas City, MO 64110, USA; Department of Anatomy and Cell Biology, Kansas University Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA. Electronic address:


The eukaryotic cell cycle, driven by both transcriptional and posttranslational mechanisms, is the central molecular oscillator underlying tissue growth throughout animals. Although genome-wide studies have investigated cell-cycle-associated transcription in unicellular systems, global patterns of periodic transcription in multicellular tissues remain largely unexplored. Here we define the cell-cycle-associated transcriptome of the developing Drosophila wing epithelium and compare it with that of cultured Drosophila S2 cells, revealing a core set of periodic genes and a surprising degree of context specificity in periodic transcription. We further employ RNAi-mediated phenotypic profiling to define functional requirements for more than 300 periodic genes, with a focus on those required for cell proliferation in vivo. Finally, we investigate uncharacterized genes required for interkinetic nuclear migration. Combined, these findings provide a global perspective on cell-cycle control in vivo, and they highlight a critical need to understand the context-specific regulation of cell proliferation.

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