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Electrophoresis. 1989 Mar;10(3):186-9.

A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism.

Author information

1
Institut de Pathologie Moléculaire, Institut National de la Santé et de la Recherche Médicale, Paris, France.

Abstract

A rapid DNA extraction procedure and random primer labelling of Escherichia coli ribosomal RNA with cloned reverse transcriptase have been used to establish a simple and highly sensitive method for studying ribosomal DNA polymorphism in bacteria. Examples of inter- and intraspecies differentiation of bacteria are given and potential applications in bacterial epidemiology and taxonomy are discussed.

PMID:
2468482
DOI:
10.1002/elps.1150100306
[Indexed for MEDLINE]

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