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J Hered. 2014 Jul-Aug;105(4):576-582. Epub 2014 Mar 28.

Defining the Role of prolamin-box binding factor1 Gene During Maize Domestication.

Author information

1
From the Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun Nandajie, Beijing 100081, China (Lang); the Department of Genetics, University of Wisconsin-Madison, 425 Henry Mall, Madison, WI 53706 (Wills, Lemmon, Shannon, and Doebley); the Department of Biomedical Sciences, Cornell University, T8 016D Veterinary Research Tower, Ithaca, NY 14853 (Shannon); the Computational Biology Service Unit, Cornell University, 620 Rhodes Hall, Ithaca, NY 14853 (Bukowski); Waksman Institute of Microbiology, Rutgers University, 190 Frelinghuysen Road, Piscataway, NJ 08854 (Wu and Messing); and the Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, CAS, 300 Feng Lin Road, Shanghai 200032, China (Wu).
2
From the Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun Nandajie, Beijing 100081, China (Lang); the Department of Genetics, University of Wisconsin-Madison, 425 Henry Mall, Madison, WI 53706 (Wills, Lemmon, Shannon, and Doebley); the Department of Biomedical Sciences, Cornell University, T8 016D Veterinary Research Tower, Ithaca, NY 14853 (Shannon); the Computational Biology Service Unit, Cornell University, 620 Rhodes Hall, Ithaca, NY 14853 (Bukowski); Waksman Institute of Microbiology, Rutgers University, 190 Frelinghuysen Road, Piscataway, NJ 08854 (Wu and Messing); and the Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, CAS, 300 Feng Lin Road, Shanghai 200032, China (Wu). jdoebley@wisc.ed.

Abstract

The prolamin-box binding factor1 (pbf1) gene encodes a transcription factor that controls the expression of seed storage protein (zein) genes in maize. Prior studies show that pbf1 underwent selection during maize domestication although how it affected trait change during domestication is unknown. To assay how pbf1 affects phenotypic differences between maize and teosinte, we compared nearly isogenic lines (NILs) that differ for a maize versus teosinte allele of pbf1 Kernel weight for the teosinte NIL (162mg) is slightly but significantly greater than that for the maize NIL (156mg). RNAseq data for developing kernels show that the teosinte allele of pbf1 is expressed at about twice the level of the maize allele. However, RNA and protein assays showed no difference in zein profile between the two NILs. The lower expression for the maize pbf1 allele suggests that selection may have favored this change; however, how reduced pbf1 expression alters phenotype remains unknown. One possibility is that pbf1 regulates genes other than zeins and thereby is a domestication trait. The observed drop in seed weight associated with the maize allele of pbf1 is counterintuitive but could represent a negative pleiotropic effect of selection on some other aspect of kernel composition.

KEYWORDS:

RNAseq ; kernel ; selection ; teosinte

PMID:
24683184
PMCID:
PMC6373496
DOI:
10.1093/jhered/esu019
[Indexed for MEDLINE]
Free PMC Article

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