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Colloids Surf B Biointerfaces. 2014 May 1;117:354-9. doi: 10.1016/j.colsurfb.2014.02.052. Epub 2014 Mar 13.

Anticancer activity of Moringa oleifera mediated silver nanoparticles on human cervical carcinoma cells by apoptosis induction.

Author information

1
Interdisciplinary School of Indian System of Medicine, SRM University, Kattankulathur 603203, Tamil Nadu, India.
2
Interdisciplinary School of Indian System of Medicine, SRM University, Kattankulathur 603203, Tamil Nadu, India; Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India.
3
Interdisciplinary School of Indian System of Medicine, SRM University, Kattankulathur 603203, Tamil Nadu, India; National Facility for Tribal and Herbal Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India. Electronic address: gpdubey131@gmail.com.

Abstract

Silver nanomaterial plays a crucial role in the growing field of nanotechnology as there is an increasing commercial demand for silver nanoparticles (AgNPs) owing to their wide biological applications. The present investigation aims at developing anti-cancerous colloidal silver using Moringa olifera stem bark extract. Electron and atomic force microscopic images were taken to analyze the surface morphology of the synthesized AgNPs. The effects of synthesized AgNPs were tested against human cervical carcinoma cells (HeLa) and cell morphology was further evaluated using 4,6-diamidino-2-phenylindole (DAPI) staining. The efficiency of green synthesized AgNPs was studied with the help of fluorescence activated cell sorting (FACS) and was shown to induce apoptosis through reactive oxygen species (ROS) generation in HeLa cells.

KEYWORDS:

Apoptosis; Electron microscopy; Reactive oxygen species; Silver nanoparticles; Surface plasmon resonance

PMID:
24681047
DOI:
10.1016/j.colsurfb.2014.02.052
[Indexed for MEDLINE]

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