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Cryobiology. 2014 Jun;68(3):395-404. doi: 10.1016/j.cryobiol.2014.03.008. Epub 2014 Mar 27.

Effects of glycerol on apoptotic signaling pathways during boar spermatozoa cryopreservation.

Author information

1
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, PR China. Electronic address: zengchj@sicau.edu.cn.
2
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan Province 625014, PR China.

Abstract

Artificial insemination (AI) with post-thawed boar spermatozoa results in low farrowing rates and reduced litter sizes mainly due to cryoinjury or damages to spermatozoa during cryopreservation. Low viability and motility of post-thawed boar spermatozoa are highly associated with apoptosis during cryopreservation. Although glycerol is widely used a cryoprotectant (CPA) for boar spermatozoa cryopreservation, the mechanism and relationship between glycerol and apoptosis-related gene expression needs to be clarified. In this study, we treated boar spermatozoa with different concentrations of glycerol in lactose egg yolk (LEY) extender to evaluate the apoptosis-related gene expression and protease activities of caspases. These results show that: (1) low concentrations of glycerol (2% and 3%) were more suitable for boar spermatozoa cryopreservation; (2) apoptosis-related genes involved in intrinsic mitochondrial and extrinsic death receptor apoptotic signaling pathways were widely expressed in different concentrations of glycerol treated boar spermatozoa; (3) there was a significant positive correlation (r=0.840, P=0.037) between the percentage of Annexin V(+)/PI(+) staining spermatozoa and caspase-6/9 protease activity. In conclusion, 2% and 3% glycerol have the best anti-apoptotic effects, and the expression of Fas/FasL and Bcl-2/Bax have a strong correlation with spermatozoa parameters.

KEYWORDS:

Apoptosis; Boar spermatozoa; Cryopreservation; Glycerol; Signaling pathway

PMID:
24680861
DOI:
10.1016/j.cryobiol.2014.03.008
[Indexed for MEDLINE]

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