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J Tissue Eng Regen Med. 2016 Dec;10(12):1012-1020. doi: 10.1002/term.1886. Epub 2014 Mar 27.

Anchoring a cytoactive factor in a wound bed promotes healing.

Author information

1
Department of Chemistry, University of Wisconsin-Madison, WI, USA.
2
Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, WI, USA.
3
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, WI, USA.
4
Department of Surgical and Radiological Sciences, School of Veterinary Medicine and Department of Ophthalmology and Vision Science, School of Medicine, University of California, Davis, CA, USA.
5
Department of Biochemistry, University of Wisconsin-Madison, WI, USA.

Abstract

Wound healing is a complex process that requires the intervention of cytoactive factors. The one-time application of soluble factors to a wound bed does not maintain a steady, sufficient concentration. Here we investigated the benefits of anchoring a factor in a wound bed via a tether to endogenous collagen. We used a collagen-mimetic peptide (CMP) as a pylon. The CMP binds to damaged but not intact collagen and thus localizes a pendant cytoactive factor in the regions of a wound bed that require intervention. As a model factor, we chose substance P, a peptide of the tachykinin family that promotes wound healing. Using splinted wounds in db/db mice, we found that the one-time application of a CMP-substance P conjugate enhances wound healing compared to unconjugated substance P and other controls. Specifically, all 16 wounds treated with the conjugate closed more thoroughly and, did so with extensive re-epithelialization and mitigated inflammatory activity. These data validate a simple and general strategy for re-engineering wound beds by the integration of beneficial cytoactive factors.

KEYWORDS:

Mus musculus; Substance P; collagen; extracellular matrix; peptide; synthetic biology

PMID:
24677775
PMCID:
PMC4219926
DOI:
10.1002/term.1886
[Indexed for MEDLINE]
Free PMC Article

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