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Aesthet Surg J. 2014 Mar;34(3):438-47. doi: 10.1177/1090820X14524416.

Autologous fat processing via the Revolve system: quality and quantity of fat retention evaluated in an animal model.

Author information

1
Dr Ansorge is a Senior Product Development Engineer, Dr Leamy is a Staff Scientist, Ms Roesch is a Senior Clinical Scientist, Mr Barere is a Director of Product Development, and Dr Connor is a Senior Director from the Department of Research and Development, LifeCell Corporation, Bridgewater, New Jersey.

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Abstract

BACKGROUND:

Currently, fat graft viability and retention cannot be reliably predicted. The reasons for this variability are not fully understood, although fat processing has been implicated.

OBJECTIVES:

The authors compare the in vitro quantity and in vivo fat retention from lipoaspirate processed by the Revolve system (LifeCell, Bridgewater, New Jersey) compared with centrifugation and decantation.

METHODS:

Ten patients were enrolled in this prospective study. Lipoaspirate from each patient was processed by each of 3 methods: decantation, centrifugation, and the Revolve system. Biochemical characteristics and free oil, adipose, and aqueous phases of the processed fats were determined. Fat grafts were implanted in nude mice; volume retention and quality of the fat grafts were evaluated after 28 days. Viability of retained fat was demonstrated by intact adipocytes and neovascularization on histology.

RESULTS:

Of the 10 patients, 9 were women and 1 was a man. Mean patient age was 40.7 ± 8.9 years (range, 30-55 years). Fat tissue obtained from all methods had good physiological properties with neutral pH and isotonic salt concentrations. The Revolve system yielded significantly less blood cell debris, a higher percentage of adipose tissue, and a lower percentage of free oil compared with the other 2 methods. Fat tissue retention from Revolve samples was significantly higher (73.2%) than that from decanted samples (37.5%) and similar to that from centrifuged samples (67.7%).

CONCLUSIONS:

The Revolve system produced physiologically compatible, preinjection fat with reduced contaminants and free oil in conjunction with high fat content. In an animal model, volume retention of Revolve-processed fat grafts was significantly greater than decanted samples. The Revolve system presents a fat-processing option that was less time-consuming, easier to use, and more efficient in this study than standard centrifugation or decantation.

KEYWORDS:

Revolve system; adipose tissue; animal model; autologous fat processing; centrifugation; decantation; fat graft viability; fat retention; lipoaspiration

PMID:
24676414
DOI:
10.1177/1090820X14524416
[Indexed for MEDLINE]
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