Format

Send to

Choose Destination
Cell Cycle. 2014;13(11):1749-55. doi: 10.4161/cc.28653. Epub 2014 Mar 26.

Promyelocytic leukemia bodies tether to early endosomes during mitosis.

Author information

1
Department of Microbiology and Department of Medical Biochemistry; Oslo University Hospital and University of Oslo; Oslo, Norway.
2
Department of Biochemistry; Institute for Cancer Research; The Norwegian Radium Hospital; Oslo University Hospital; Oslo, Norway.

Abstract

During mitosis the nuclear envelope breaks down, leading to potential interactions between cytoplasmic and nuclear components. PML bodies are nuclear structures with tumor suppressor and antiviral functions. Early endosomes, on the other hand, are cytoplasmic vesicles involved in transport and growth factor signaling. Here we demonstrate that PML bodies form stable interactions with early endosomes immediately following entry into mitosis. The 2 compartments remain stably associated throughout mitosis and dissociate in the cytoplasm of newly divided daughter cells. We also show that a minor subset of PML bodies becomes anchored to the mitotic spindle poles during cell division. The study demonstrates a stable mitosis-specific interaction between a cytoplasmic and a nuclear compartment.

KEYWORDS:

CyPNs; MAPPs; PML; PML bodies; Rab5; centrosome; endocytosis; endosomes; mitosis

PMID:
24675887
PMCID:
PMC4111721
DOI:
10.4161/cc.28653
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Taylor & Francis Icon for PubMed Central
Loading ...
Support Center