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Sci Rep. 2014 Mar 28;4:4495. doi: 10.1038/srep04495.

Unambiguous observation of shape effects on cellular fate of nanoparticles.

Author information

1
Department of Physics and Centre for Quantum Coherence, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
2
School of Biomedical Sciences, Faculty of medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
3
Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan.
4
Laboratory of Synthetic Nanochemistry, Institute of Organic Chemistry and Biochemistry AS CR, v.v.i., Flemingovo nam. 2, 166 10, Prague 6, Czech Republic.
5
1] Department of Physics and Centre for Quantum Coherence, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong [2] The Chinese University of Hong Kong ShenZhen Research Institute, ShenZhen, China.

Abstract

Cellular fate of nanoparticles is vital to application of nanoparticles to cell imaging, bio-sensing, drug delivery, suppression of drug resistance, gene delivery, and cytotoxicity analysis. However, the current studies on cellular fate of nanoparticles have been controversial due to complications of interplay between many possible factors. By well-controlled experiments, we demonstrated unambiguously that the morphology of nanoparticles independently determined their cellular fate. We found that nanoparticles with sharp shapes, regardless of their surface chemistry, size, or composition, could pierce the membranes of endosomes that carried them into the cells and escape to the cytoplasm, which in turn significantly reduced the cellular excretion rate of the nanoparticles. Such features of sharp-shaped nanoparticles are essential for drug delivery, gene delivery, subcellular targeting, and long-term tracking. This work opens up a controllable, purely geometrical and hence safe, degree of freedom for manipulating nanoparticle-cell interaction, with numerous applications in medicine, bio-imaging, and bio-sensing.

PMID:
24675513
PMCID:
PMC3968459
DOI:
10.1038/srep04495
[Indexed for MEDLINE]
Free PMC Article

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