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Ecotoxicol Environ Saf. 2014 Jun;104:127-31. doi: 10.1016/j.ecoenv.2014.01.039. Epub 2014 Mar 25.

Uptake of a cyanotoxin, β-N-methylamino-L-alanine, by wheat (Triticum aestivum).

Author information

1
Technische Universität Berlin, Institute of Ecology, Department Ecological Impact Research and Ecotoxicology, Ernst-Reuter-Platz 1, 10587 Berlin, Germany. Electronic address: v.contardojara@tu-berlin.de.
2
Technische Universität Berlin, Institute of Ecology, Department Ecological Impact Research and Ecotoxicology, Ernst-Reuter-Platz 1, 10587 Berlin, Germany. Electronic address: Torsten@Schwanemann.de.
3
Technische Universität Berlin, Institute of Ecology, Department Ecological Impact Research and Ecotoxicology, Ernst-Reuter-Platz 1, 10587 Berlin, Germany. Electronic address: stephan.pflugmacher@tu-berlin.de.

Abstract

In order to study the uptake of the cyanobacterial neurotoxin β-N-methylamino-l-alanine (BMAA) into the crop plant Triticum aestivum during germination and primary growth imbibed grains and 7-day-old seedlings were irrigated with 100 and 1000µg l(-1) BMAA for 4 days and 100µg l(-1) BMAA for 28 days. Content of derivatized free and protein-associated BMAA in seedlings, root and shoot tissue, respectively, were analyzed by LC-MS/MS. Free BMAA was only detected in seedlings exposed to 1000µg l(-1) BMAA, whereas protein-associated BMAA was found at both exposure concentrations. Irrigation with 100µgl(-1) BMAA led to an uptake of the neurotoxin into roots and shoots and to immediate protein-association. In roots, protein-associated BMAA was detectable after 5 days with peaking amounts after 14 days. Longer exposure did not cause further accumulation in roots. In contrast, protein-associated BMAA was detected in shoot samples after only 1 day. In shoots the highest amounts of protein-associated BMAA were found after 28 days. In turn, in both plant compartments free BMAA was below the measurable concentration.

KEYWORDS:

Cyanobacterial neurotoxin; Protein-association; Triticum aestivum; Up-take; β-N-methylamino-l-alanine

PMID:
24675440
DOI:
10.1016/j.ecoenv.2014.01.039
[Indexed for MEDLINE]

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