Format

Send to

Choose Destination
Autophagy. 2014 May;10(5):878-88. doi: 10.4161/auto.28279. Epub 2014 Mar 24.

OsATG7 is required for autophagy-dependent lipid metabolism in rice postmeiotic anther development.

Author information

1
Department of Applied Biological Science; Tokyo University of Science; Noda, Chiba Japan; Research Institute for Science and Technology; Tokyo University of Science; Noda, Chiba Japan; School of Bioscience and Biotechnology; Tokyo University of Technology; Hachioji, Tokyo Japan.
2
Department of Applied Biological Science; Tokyo University of Science; Noda, Chiba Japan.
3
Plant Genetics Laboratory; National Institute of Genetics; Mishima, Shizuoka Japan.
4
Department of Chemical Biological Science; Faculty of Science; Japan Women's University; Bunkyo-ku, Tokyo Japan.
5
Faculty of Bioscience; Nagahama Institute of Bio-Science and Technology; Shiga, Japan.
6
Metabolomic Function Research Group; RIKEN Center for Sustainable Resource Science; Yokohama, Kanagawa Japan.
7
Environmental Stress Research Unit; Division of Plant Sciences; National Institute of Agrobiological Sciences; Tsukuba, Ibaraki Japan.
8
Department of Applied Plant Science; Graduate School of Agricultural Sciences; Tohoku University; Sendai, Miyagi, Japan.
9
Agrogenomics Research Center; National Institute of Agrobiological Sciences; Tsukuba, Ibaraki Japan.
10
Research Institute for Science and Technology; Tokyo University of Science; Noda, Chiba Japan; Department of Biological Science and Technology; Tokyo University of Science; Katsushika, Tokyo Japan.
11
Metabolomic Function Research Group; RIKEN Center for Sustainable Resource Science; Yokohama, Kanagawa Japan; Graduate School of Pharmaceutical Sciences; Chiba University; Chiba, Chiba Japan.
12
Department of Applied Biological Science; Tokyo University of Science; Noda, Chiba Japan; Research Institute for Science and Technology; Tokyo University of Science; Noda, Chiba Japan.

Abstract

In flowering plants, the tapetum, the innermost layer of the anther, provides both nutrient and lipid components to developing microspores, pollen grains, and the pollen coat. Though the programmed cell death of the tapetum is one of the most critical and sensitive steps for fertility and is affected by various environmental stresses, its regulatory mechanisms remain mostly unknown. Here we show that autophagy is required for the metabolic regulation and nutrient supply in anthers and that autophagic degradation within tapetum cells is essential for postmeiotic anther development in rice. Autophagosome-like structures and several vacuole-enclosed lipid bodies were observed in postmeiotic tapetum cells specifically at the uninucleate stage during pollen development, which were completely abolished in a retrotransposon-insertional OsATG7 (autophagy-related 7)-knockout mutant defective in autophagy, suggesting that autophagy is induced in tapetum cells. Surprisingly, the mutant showed complete sporophytic male sterility, failed to accumulate lipidic and starch components in pollen grains at the flowering stage, showed reduced pollen germination activity, and had limited anther dehiscence. Lipidomic analyses suggested impairment of editing of phosphatidylcholines and lipid desaturation in the mutant during pollen maturation. These results indicate a critical involvement of autophagy in a reproductive developmental process of rice, and shed light on the novel autophagy-mediated regulation of lipid metabolism in eukaryotic cells.

KEYWORDS:

anther; autophagy; male sterility; pollen development; rice

PMID:
24674921
PMCID:
PMC5119067
DOI:
10.4161/auto.28279
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Taylor & Francis Icon for PubMed Central
Loading ...
Support Center