Format

Send to

Choose Destination
Neurosci Lett. 2014 May 1;567:19-23. doi: 10.1016/j.neulet.2014.03.018. Epub 2014 Mar 24.

Involvement of amygdaloid neuropeptide Y in the anxiolytic effects of acupuncture during ethanol withdrawal in rats.

Author information

1
Department of Pharmacology, Mudanjiang Medical University, Mudanjiang, China.
2
College of Oriental Medicine, Daegu Haany University, Daegu, Republic of Korea.
3
Department of Preventive Medicine, Hygiene, Yanbian University, Yanji, China.
4
College of Oriental Medicine, Daegu Haany University, Daegu, Republic of Korea. Electronic address: dlqhdgy@dhu.ac.kr.
5
Department of Pharmacology, Mudanjiang Medical University, Mudanjiang, China; College of Oriental Medicine, Daegu Haany University, Daegu, Republic of Korea. Electronic address: zhao_rongjie@yahoo.com.

Abstract

The role of neuropeptide Y (NPY) in the central nucleus of amygdala (CeA) in the preventive effects of acupuncture against ethanol withdrawal-induced anxiety was investigated. Rats were treated with 3g/kg/day of ethanol for 28 days, followed by 3 days of withdrawal. Bilateral acupuncture treatment at HT7 (Shen-Men), PC6 (Nei-Guan) or a non-acupoint was respectively added to the rats during the withdrawal once a day for three days. Enzyme-linked immunosorbent assays and real-time polymerase chain reaction analyses showed there was a significant decrease in NPY protein and mRNA expression in the CeA during ethanol withdrawal, which was reversed by acupuncture at HT7 but neither at PC6 nor at a non-acupoint. Acupuncture at HT7 also greatly inhibited the decrease in cAMP response element-binding protein (CREB) phosphorylation in the CeA. In elevated plus maze tests, a selective NPY Y1 receptor antagonist BIBP 3226 into the CeA before the acupuncture abolished almost completely the anxiolytic effect of acupuncture at HT7. These results suggest that acupuncture at HT7 rescues the depletion of amygdaloid NPY and reverses the decrease in CREB phosphorylation to produce anxiolytic effects during ethanol withdrawal.

KEYWORDS:

Acupuncture; Amygdala; Ethanol withdrawal; Neuropeptide Y; cAMP response element-binding protein

PMID:
24674772
DOI:
10.1016/j.neulet.2014.03.018
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center