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BMC Genomics. 2014 Mar 27;15:240. doi: 10.1186/1471-2164-15-240.

Deleted copy number variation of Hanwoo and Holstein using next generation sequencing at the population level.

Author information

1
Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea. dhyoon@knu.ac.kr.

Abstract

BACKGROUND:

Copy number variation (CNV), a source of genetic diversity in mammals, has been shown to underlie biological functions related to production traits. Notwithstanding, there have been few studies conducted on CNVs using next generation sequencing at the population level.

RESULTS:

Illumina NGS data was obtained for ten Holsteins, a dairy cattle, and 22 Hanwoo, a beef cattle. The sequence data for each of the 32 animals varied from 13.58-fold to almost 20-fold coverage. We detected a total of 6,811 deleted CNVs across the analyzed individuals (average length = 2732.2 bp) corresponding to 0.74% of the cattle genome (18.6 Mbp of variable sequence). By examining the overlap between CNV deletion regions and genes, we selected 30 genes with the highest deletion scores. These genes were found to be related to the nervous system, more specifically with nervous transmission, neuron motion, and neurogenesis. We regarded these genes as having been effected by the domestication process. Further analysis of the CNV genotyping information revealed 94 putative selected CNVs and 954 breed-specific CNVs.

CONCLUSIONS:

This study provides useful information for assessing the impact of CNVs on cattle traits using NGS at the population level.

PMID:
24673797
PMCID:
PMC4051123
DOI:
10.1186/1471-2164-15-240
[Indexed for MEDLINE]
Free PMC Article

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