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Methods Mol Biol. 2014;1144:19-29. doi: 10.1007/978-1-4939-0428-0_2.

Herpes simplex virus growth, preparation, and assay.

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1
Section of Applied Microbiology and Pathology, Department of Life Sciences and Biotechnology (SVeB), University of Ferrara, Via Luigi Borsari 46, Ferrara, 44121, Italy, mcy@unife.it.

Abstract

In order to study the biology of herpes simplex virus or to use it as a vector in gene therapy, it is necessary to grow the virus and to prepare virus stocks. Many different protocols are available from different research groups working with herpes simplex virus type 1 or 2 (HSV-1 or HSV-2). This chapter describes the procedures used in our laboratory.

PMID:
24671674
DOI:
10.1007/978-1-4939-0428-0_2
[Indexed for MEDLINE]
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