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J Immunol Methods. 1989 Mar 31;118(2):179-86.

Immunoblot fingerprinting Aspergillus fumigatus.

Author information

1
Department of Medical Microbiology, St. Bartholomew's Hospital, London, U.K.

Abstract

A new technique for typing Aspergillus fumigatus is presented. This is based on immunoblot fingerprinting each isolate against a rabbit hyperimmune antiserum raised against A. fumigatus NCTC 2109. All isolates were typable and reproducibility for the 16 antigenic bands which formed the basis of the system was excellent. Discrimination was better than silver staining and revealed 11 types among the 21 isolates from eight patients with an aspergilloma. Each aspergilloma could be due to either a single or multiple types.

PMID:
2466902
DOI:
10.1016/0022-1759(89)90004-5
[Indexed for MEDLINE]

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