Send to

Choose Destination
J Integr Med. 2014 Mar;12(2):115-20. doi: 10.1016/S2095-4964(14)60011-X.

Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: a light microscopic evaluation.

Author information

Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Clinical Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran; E-mail:



Ulcerative colitis is a chronically recurrent inflammatory bowel disease of unknown origin. In the present study, the effect of ginger (rhizome of Zingiber officinale Roscoe) volatile oil on a rat model of colitis was evaluated.


Volatile oil of ginger with doses of 100, 200, and 400 mg/kg, prednisolone (4 mg/kg), or vehicle were administered orally to groups of male Wistar rats (n = 6) for 5 d. Animals were randomly divided into 6 groups, each group consisting of 6 rats. Colitis was induced by intracolonic instillation of 2 mL of 4% (v/v) acetic acid solution. All rats were sacrificed 24 h later and the tissue injuries were assessed macroscopically and histopathologically.


Ginger volatile oil with all doses reduced colon weight/length ratio (P < 0.01) and the effects were similar to the reference drugs. Higher oral doses of volatile oil (200 and 400 mg/kg) reduced ulcer severity (P < 0.05 and P < 0.01), ulcer area (P < 0.01) and ulcer index (P < 0.01). On the other hand, evaluation of microscopic scores showed that the dose of 400 mg/kg of volatile oil was effective to reduce inflammation severity (P < 0.01) and inflammation extent (P < 0.05) compared to the control group.


It is concluded that ginger volatile oil could effectively reduce symptoms of experimental colitis in a dose-dependent manner.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center