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Tissue Barriers. 2013 Jul 1;1(3):e24517. doi: 10.4161/tisb.24517. Epub 2013 Apr 10.

Claudin family members exhibit unique temporal and spatial expression boundaries in the chick embryo.

Author information

  • 1Department of Human Genetics; McGill University; Montréal, QC Canada ; Research Institute of the McGill University Health Centre; Montréal, QC Canada.
  • 2Department of Human Genetics; McGill University; Montréal, QC Canada ; Research Institute of the McGill University Health Centre; Montréal, QC Canada ; Department of Pediatrics; McGill University; Montréal, QC Canada.

Abstract

The claudin family of proteins are integral components of tight junctions and are responsible for determining the ion specificity and permeability of paracellular transport within epithelial and endothelial cell layers. Several members of the claudin family have been shown to be important during embryonic development and morphogenesis. However, detailed embryonic expression patterns have been described for only a few claudins. Here, we provide a phylogenetic analysis of the chicken claudins and a comprehensive analysis of their mRNA expression profiles. We found that claudin family members exhibit both overlapping and unique expression patterns throughout development. Especially striking were the distinct expression boundaries observed between neural and non-neural ectoderm, as well as within ectodermal derivatives. Claudins were also expressed in endodermally-derived tissues, including the anterior intestinal portal, pharynx, lung and pancreas and in mesodermally derived tissues such as the kidney, gonad and heart. The overlapping zones of claudin expression observed in the chick embryo may confer distinct domains of ion permeability within the early epiblast and in epithelial, mesodermal and endothelial derivatives that may ultimately influence embryonic patterning and morphogenesis during development.

KEYWORDS:

boundaries; chick development; claudin; expression; tight junction

PMID:
24665397
PMCID:
PMC3875640
DOI:
10.4161/tisb.24517
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