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Mediators Inflamm. 2014;2014:472625. doi: 10.1155/2014/472625. Epub 2014 Feb 10.

Interleukin-1 receptors are differentially expressed in normal and psoriatic T cells.

Author information

1
Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, Szeged 6720, Hungary.
2
Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, Szeged 6720, Hungary ; Dermatological Research Group of the Hungarian Academy of Sciences, University of Szeged, Korányi fasor 6, Szeged 6720, Hungary.
3
Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, Szeged 6720, Hungary ; Department of Dermatology, Venereology and Oncodermatology, University of Pécs, Kodály Z. u. 20, Pécs 7624, Hungary.

Abstract

This study was carried out to examine the possible role of interleukin-1 (IL-1) in the functional insufficiency of regulatory T cells in psoriasis, by comparing the expression of IL-1 receptors on healthy control and psoriatic T cells. Patients with moderate-to-severe chronic plaque psoriasis and healthy volunteers, matched in age and sex, were selected for all experiments. CD4(+)CD25(-) effector and CD4(+)CD25(+)CD127(low) regulatory T cells were separated and used for the experiments. Expression of the mRNA of IL-1 receptors (IL-1R1, IL-1R2, and sIL-1R2) was determined by quantitative real-time RT-PCR. Cell surface IL-1 receptor expression was assessed by flow cytometry. Relative expression of the signal transmitting IL-1 receptor type 1 (IL-1R1) mRNA is higher in resting psoriatic effector and regulatory T cells, and activation induces higher IL-1R1 protein expression in psoriatic T cells than in healthy cells. Psoriatic regulatory and effector T cells express increased mRNA levels of the decoy IL-1 receptors (IL-1R2 and sIL-1R2) upon activation compared to healthy counterparts. Psoriatic T cells release slightly more sIL-1R2 into their surrounding than healthy T cells. In conclusion, changes in the expression of IL-1 receptors in psoriatic regulatory and effector T cells could contribute to the pathogenesis of psoriasis.

PMID:
24665164
PMCID:
PMC3934444
DOI:
10.1155/2014/472625
[Indexed for MEDLINE]
Free PMC Article

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