Format

Send to

Choose Destination
Cell Res. 2014 May;24(5):513-31. doi: 10.1038/cr.2014.35. Epub 2014 Mar 25.

Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus.

Author information

1
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, 320 Yueyang Road, Shanghai 200031, China.
2
Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.
3
Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, China.

Erratum in

  • Cell Res. 2014 Sep;24(9):1150.

Abstract

The human 8q24 gene desert contains multiple enhancers that form tissue-specific long-range chromatin loops with the MYC oncogene, but how chromatin looping at the MYC locus is regulated remains poorly understood. Here we demonstrate that a long noncoding RNA (lncRNA), CCAT1-L, is transcribed specifically in human colorectal cancers from a locus 515 kb upstream of MYC. This lncRNA plays a role in MYC transcriptional regulation and promotes long-range chromatin looping. Importantly, the CCAT1-L locus is located within a strong super-enhancer and is spatially close to MYC. Knockdown of CCAT1-L reduced long-range interactions between the MYC promoter and its enhancers. In addition, CCAT1-L interacts with CTCF and modulates chromatin conformation at these loop regions. These results reveal an important role of a previously unannotated lncRNA in gene regulation at the MYC locus.

Comment in

PMID:
24662484
PMCID:
PMC4011346
DOI:
10.1038/cr.2014.35
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center