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Biochem Biophys Res Commun. 2014 Apr 18;446(4):1047-52. doi: 10.1016/j.bbrc.2014.03.053. Epub 2014 Mar 21.

Discovery of Apo-A1 as a potential bladder cancer biomarker by urine proteomics and analysis.

Author information

1
Cancer Immunity Research Laboratory, Tianjin Institute of Urology, Second Hospital of Tianjin Medical University, Tianjin, China. Electronic address: lichangying80@gmail.com.
2
Institute of Basic Medicine, Hebei United University, Tangshan, China.
3
Cancer Immunity Research Laboratory, Tianjin Institute of Urology, Second Hospital of Tianjin Medical University, Tianjin, China.
4
Library of Tianjin Medical University, Tianjin, China.
5
Cancer Immunity Research Laboratory, Tianjin Institute of Urology, Second Hospital of Tianjin Medical University, Tianjin, China. Electronic address: jwchang_cirl@163.com.

Abstract

Bladder cancer is clinically characterized by high recurrent rate and poor prognosis and thereby patients need regular re-examinations which are invasive, unpleasant, and expensive. A noninvasive and less expensive method for detecting and monitoring bladder cancer would thus be advantageous. In this study, by using the two-dimensional electrophoresis (2-DE) approach with subsequent mass spectrometry (MS), we demonstrated the increased expression of apolipoprotein-A1 (Apo-A1) in individual urine from patients with bladder cancer, which was confirmed by Western blot results. A further analysis of the urinary Apo-A1 levels by an enzyme-linked immunosorbent assay yielded results that were consistent with the Western blot, and suggested Apo-A1 could provide diagnostic utility to distinguish patients with bladder cancer from healthy controls at 19.21 ng/ml. Further validation assay in a larger number of urine samples (n=379) showed that Apo-A1 could be used as a biomarker to diagnosis bladder cancer with a sensitivity and specificity of 89.2% and 84.6% respectively. Moreover, the application of exfoliative urinary cytology in combination with the urine Apo-A1 detection could significantly increased the sensitivity in detecting bladder cancer. Our data showed a significant relationship of expressed Apo-A1 was established between bladder cancer and normal controls. Apo-A1 could be a potential biomarker for the diagnosis of bladder cancer.

KEYWORDS:

Apolipoprotein-A1; Biomarker; Bladder cancer; Diagnosis; Proteomics

PMID:
24661883
DOI:
10.1016/j.bbrc.2014.03.053
[Indexed for MEDLINE]
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