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J Exp Bot. 2014 Jun;65(9):2243-56. doi: 10.1093/jxb/eru089. Epub 2014 Mar 22.

Transcriptional and hormonal regulation of petal and stamen development by STAMENLESS, the tomato (Solanum lycopersicum L.) orthologue to the B-class APETALA3 gene.

Author information

1
Groupe de Recherche en Physiologie végétale, Earth and Life Institute, Université catholique de Louvain, Croix du Sud 4-5 bte L7.07.13, B-1348 Louvain-la-Neuve, Belgium muriel.quinet@uclouvain.be.
2
Groupe de Recherche en Physiologie végétale, Earth and Life Institute, Université catholique de Louvain, Croix du Sud 4-5 bte L7.07.13, B-1348 Louvain-la-Neuve, Belgium.
3
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, Prague 6, 16502, Czech Republic.
4
Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, 04120 Almería, Spain.

Abstract

Four B-class MADS box genes specify petal and stamen organ identities in tomato. Several homeotic mutants affected in petal and stamen development were described in this model species, although the causal mutations have not been identified for most of them. In this study we characterized a strong stamenless mutant in the tomato Primabel cultivar (sl-Pr), which exhibited homeotic conversion of petals into sepals and stamens into carpels and we compared it with the stamenless mutant in the LA0269 accession (sl-LA0269). Genetic complementation analysis proved that both sl mutants were allelic. Sequencing revealed point mutations in the coding sequence of the Tomato APETALA3 (TAP3) gene of the sl-Pr genome, which lead to a truncated protein, whereas a chromosomal rearrangement in the TAP3 promoter was detected in the sl-LA0269 allele. Moreover, the floral phenotype of TAP3 antisense plants exhibited identical homeotic changes to sl mutants. These results demonstrate that SL is the tomato AP3 orthologue and that the mutant phenotype correlated to the SL silencing level. Expression analyses showed that the sl-Pr mutation does not affect the expression of other tomato B-class genes, although SL may repress the A-class gene MACROCALYX. A partial reversion of the sl phenotype by gibberellins, gene expression analysis, and hormone quantification in sl flowers revealed a role of phytohormones in flower development downstream of the SL gene. Together, our results indicated that petal and stamen identity in tomato depends on gene-hormone interactions, as mediated by the SL gene.

KEYWORDS:

APETALA3; B-class gene; STAMENLESS; Solanum lycopersicum; flower morphogenesis; hormone regulation; tomato.

PMID:
24659487
PMCID:
PMC4036497
DOI:
10.1093/jxb/eru089
[Indexed for MEDLINE]
Free PMC Article

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