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Comp Biochem Physiol Part D Genomics Proteomics. 2014 Jun;10:22-9. doi: 10.1016/j.cbd.2014.02.001. Epub 2014 Mar 1.

The influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome.

Author information

1
Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; Molecular and Cellular Biology Program, Oregon State University, Corvallis, OR 97331, USA.
2
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA.
3
Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA.
4
Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.
5
Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR 97331, USA.
6
Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA; School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR 97331, USA.
7
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA; Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA. Electronic address: robert.tanguay@oregonstate.edu.

Abstract

The composition of the typical commercial diet fed to zebrafish can dramatically vary. By utilizing defined diets we sought to answer two questions: 1) How does the embryonic zebrafish transcriptome change when the parental adults are fed a commercial lab diet compared with a sufficient, defined diet (E+)? 2) Does a vitamin E-deficient parental diet (E-) further change the embryonic transcriptome? We conducted a global gene expression study using embryos from zebrafish fed a commercial (Lab), an E+ or an E- diet. To capture differentially expressed transcripts prior to onset of overt malformations observed in E- embryos at 48h post-fertilization (hpf), embryos were collected from each group at 36hpf. Lab embryos differentially expressed (p<0.01) 946 transcripts compared with the E+ embryos, and 2656 transcripts compared with the E- embryos. The differences in protein, fat and micronutrient intakes in zebrafish fed the Lab compared with the E+ diet demonstrate that despite overt morphologic consistency, significant differences in gene expression occurred. Moreover, functional analysis of the significant transcripts in the E- embryos suggested perturbed energy metabolism, leading to overt malformations and mortality. Thus, these findings demonstrate that parental zebrafish diet has a direct impact on the embryonic transcriptome.

KEYWORDS:

Defined diet; Microarray; Transcriptome; Vitamin E; Zebrafish

PMID:
24657723
PMCID:
PMC4037372
DOI:
10.1016/j.cbd.2014.02.001
[Indexed for MEDLINE]
Free PMC Article
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