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Exp Parasitol. 2014 Jun;141:21-7. doi: 10.1016/j.exppara.2014.03.007. Epub 2014 Mar 19.

Trypanocidal action of tea tree oil (Melaleuca alternifolia) against Trypanosoma evansi in vitro and in vivo used mice as experimental model.

Author information

1
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil; Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
2
Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil. Electronic address: aleksandro_ss@yahoo.com.br.
3
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
4
Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil; Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
5
Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
6
Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
7
Department of Animal Production, Universidade do Estado de Santa Catarina (UDESC), Lages, SC, Brazil.
8
Laboratory of Phytochemistry, UFSM, Santa Maria, RS, Brazil.
9
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. Electronic address: sgmonteiro@uol.com.br.

Abstract

This study aimed to evaluate the Trypanosoma evansi susceptibility to tea tree oil (TTO - Melaleuca alternifolia) and tea tree oil nanocapsules (TTO nanocapsules) in vitro and in vivo tests. In vitro, we observed a mortality curve of trypomastigotes proportional to dose, i.e., the TTO and TTO nanocapsules have trypanocidal effect. Treatment with TTO in vivo was assessed in experiments (I and II). For Experiment I, T. evansi infected mice were treated with TTO and/or combinations of essential oil with chemotherapy (diminazene aceturate - D.A.). Treatment with TTO at a dose of 1mLkg(-1) was able to extend animal longevity, but had no curative efficacy. However, when TTO was combined with D.A. a disease curative efficacy of 100% for disease was observed, a much better result than the D.A. treatment (33.3%). In Experiment II, T. evansi infected mice were treated with TTO nanocapsules with doses of 0.3, 0.6 and 0.9mLkg(-1). Animals treated with 0.9mLkg(-1) showed higher longevity however without curative effect. Active compounds present in natural products, such as M. alternifolia, may potentiate the treatment of trypanosomosis when associated with other trypanocidal drugs.

KEYWORDS:

TTO; Trypanocidal effect; Trypanosoma evansi

PMID:
24657576
DOI:
10.1016/j.exppara.2014.03.007
[Indexed for MEDLINE]

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