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Biochem Biophys Res Commun. 2014 Apr 18;446(4):863-9. doi: 10.1016/j.bbrc.2014.03.017. Epub 2014 Mar 20.

Identification of prosaposin and transgelin as potential biomarkers for gallbladder cancer using quantitative proteomics.

Author information

1
Institute of Bioinformatics, International Technology Park, Bangalore 560066, India.
2
Centre for Genomics, Molecular and Human Genetics, Jiwaji University, Gwalior 474011, India; School of Studies in Zoology, Jiwaji University, Gwalior, India.
3
Centre for Genomics, Molecular and Human Genetics, Jiwaji University, Gwalior 474011, India.
4
Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; Amrita School of Biotechnology, Amrita University, Kollam, India.
5
Cancer Hospital and Research Institute, Gwalior, India.
6
LAB SURGPATH, Mumbai, India.
7
McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Pathology, Universidad de La Frontera, CEGIN-BIOREN, Temuco, Chile.
8
Department of Pathology, Pontificia Universidad Catolica de Chile, Santiago, Chile.
9
McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
10
Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
11
Centre for Genomics, Molecular and Human Genetics, Jiwaji University, Gwalior 474011, India; School of Studies in Zoology, Jiwaji University, Gwalior, India. Electronic address: pk_tiwari@hotmail.com.

Abstract

Gallbladder cancer is an uncommon but lethal malignancy with particularly high incidence in Chile, India, Japan and China. There is a paucity of unbiased large-scale studies investigating molecular basis of gallbladder cancer. To systematically identify differentially regulated proteins in gallbladder cancer, iTRAQ-based quantitative proteomics of gallbladder cancer was carried out using Fourier transform high resolution mass spectrometry. Of the 2575 proteins identified, proteins upregulated in gallbladder cancer included several lysosomal proteins such as prosaposin, cathepsin Z and cathepsin H. Downregulated proteins included serine protease HTRA1 and transgelin, which have been reported to be downregulated in several other cancers. Novel biomarker candidates including prosaposin and transgelin were validated to be upregulated and downregulated, respectively, in gallbladder cancer using tissue microarrays. Our study provides the first large scale proteomic characterization of gallbladder cancer which will serve as a resource for future discovery of biomarkers for gallbladder cancer.

KEYWORDS:

Biomarkers; Gallbladder cancer; Prosaposin; Quantitative proteomics; Transgelin; iTRAQ

PMID:
24657443
PMCID:
PMC4696041
DOI:
10.1016/j.bbrc.2014.03.017
[Indexed for MEDLINE]
Free PMC Article
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