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Mol Cell. 2014 Apr 10;54(1):30-42. doi: 10.1016/j.molcel.2014.02.019. Epub 2014 Mar 20.

A two-step process for epigenetic inheritance in Arabidopsis.

Author information

1
Howard Hughes Medical Institute, Indiana University, Bloomington, IN 47405, USA; Department of Biology and Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA.
2
Department of Biology and Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA.
3
Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405, USA; School of Informatics and Computing, Indiana University, Bloomington, IN 47405, USA.
4
School of Informatics and Computing, Indiana University, Bloomington, IN 47405, USA.
5
Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, USA.
6
Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405, USA.
7
Department of Biology and Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA; Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405, USA.
8
Howard Hughes Medical Institute, Indiana University, Bloomington, IN 47405, USA; Department of Biology and Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA. Electronic address: cpikaard@indiana.edu.

Abstract

In Arabidopsis, multisubunit RNA polymerases IV and V orchestrate RNA-directed DNA methylation (RdDM) and transcriptional silencing, but what identifies the loci to be silenced is unclear. We show that heritable silent locus identity at a specific subset of RdDM targets requires HISTONE DEACETYLASE 6 (HDA6) acting upstream of Pol IV recruitment and siRNA biogenesis. At these loci, epigenetic memory conferring silent locus identity is erased in hda6 mutants such that restoration of HDA6 activity cannot restore siRNA biogenesis or silencing. Silent locus identity is similarly lost in mutants for the cytosine maintenance methyltransferase, MET1. By contrast, pol IV or pol V mutants disrupt silencing without erasing silent locus identity, allowing restoration of Pol IV or Pol V function to restore silencing. Collectively, these observations indicate that silent locus specification and silencing are separable steps that together account for epigenetic inheritance of the silenced state.

PMID:
24657166
PMCID:
PMC3988221
DOI:
10.1016/j.molcel.2014.02.019
[Indexed for MEDLINE]
Free PMC Article

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