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J Neuroimmunol. 2014 May 15;270(1-2):67-74. doi: 10.1016/j.jneuroim.2014.03.004. Epub 2014 Mar 12.

Presence of heat shock protein 70 in secondary lymphoid tissue correlates with stroke prognosis.

Author information

1
Functional Unit of Cerebrovascular Diseases, Hospital Clínic, Barcelona, Spain.
2
Department of Brain Ischemia and Neurodegeneration, Institute for Biomedical Research of Barcelona (IIBB), Spanish Research Council (CSIC), Barcelona, Spain; August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain.
3
Functional Unit of Cerebrovascular Diseases, Hospital Clínic, Barcelona, Spain; August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain.
4
Functional Unit of Cerebrovascular Diseases, Hospital Clínic, Barcelona, Spain; August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain; School of Medicine, University of Barcelona, Spain. Electronic address: achamorro@ub.edu.

Abstract

Heat shock protein 70 (Hsp-70) can act as a danger signal and activate immune responses. We studied the presence of Hsp-70 in lymphoid tissue and plasma of acute stroke patients and asymptomatic controls free of neurological disease. Immunofluorescence, Western blotting, qRT-PCR and flow cytometry studies were performed. Plasma Hsp-70 concentration at day 7 was similar in patients and controls, whereas patients disclosed stronger immunoreactivity to Hsp-70 in lymphoid tissue than controls. Most Hsp-70+ cells were antigen presenting cells located in T cell zones. Stronger immunoreactivity to Hsp-70 was associated with smaller infarctions and better functional outcome.

KEYWORDS:

Acute stroke; Antigen presentation; Heat shock protein; Lymphoid tissue; Neuroimmunology

PMID:
24656941
DOI:
10.1016/j.jneuroim.2014.03.004
[Indexed for MEDLINE]

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