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Cell Rep. 2014 Apr 10;7(1):269-80. doi: 10.1016/j.celrep.2014.02.030. Epub 2014 Mar 20.

Cell-type-specific circuit connectivity of hippocampal CA1 revealed through Cre-dependent rabies tracing.

Author information

1
Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697-1275, USA.
2
II. Medizinische Klinik, Klinikum rechts der Isar, Technische Universität München, 81675 Munich, Germany.
3
Systems Neurobiology Laboratories, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
4
Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697-1275, USA; Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697-2715, USA; Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697-4025, USA. Electronic address: xiangmin.xu@uci.edu.

Abstract

We developed and applied a Cre-dependent, genetically modified rabies-based tracing system to map direct synaptic connections to specific CA1 neuron types in the mouse hippocampus. We found common inputs to excitatory and inhibitory CA1 neurons from CA3, CA2, the entorhinal cortex (EC), the medial septum (MS), and, unexpectedly, the subiculum. Excitatory CA1 neurons receive inputs from both cholinergic and GABAergic MS neurons, whereas inhibitory neurons receive a great majority of inputs from GABAergic MS neurons. Both cell types also receive weaker input from glutamatergic MS neurons. Comparisons of inputs to CA1 PV+ interneurons versus SOM+ interneurons showed similar strengths of input from the subiculum, but PV+ interneurons received much stronger input than SOM+ neurons from CA3, the EC, and the MS. Thus, rabies tracing identifies hippocampal circuit connections and maps how the different input sources to CA1 are distributed with different strengths on each of its constituent cell types.

PMID:
24656815
PMCID:
PMC3998524
DOI:
10.1016/j.celrep.2014.02.030
[Indexed for MEDLINE]
Free PMC Article
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