Format

Send to

Choose Destination
Immunity. 2014 Mar 20;40(3):436-50. doi: 10.1016/j.immuni.2014.03.002.

Functional analysis via standardized whole-blood stimulation systems defines the boundaries of a healthy immune response to complex stimuli.

Author information

1
Center for Human Immunology, Institut Pasteur, 75015 Paris, France; INSERM U818, 75015 Paris, France; Laboratory of Dendritic Cell Immunobiology, Department of Immunology, Institut Pasteur, 75015 Paris, France.
2
Center for Human Immunology, Institut Pasteur, 75015 Paris, France; Center for Bioinformatics, Institut Pasteur, 75015 Paris, France.
3
Center for Human Immunology, Institut Pasteur, 75015 Paris, France.
4
INSERM U818, 75015 Paris, France; Laboratory of Dendritic Cell Immunobiology, Department of Immunology, Institut Pasteur, 75015 Paris, France.
5
Myriad Rules Based Medicine, Inc., Austin, TX 78759, USA.
6
Center for the Integration of Clinical Research, Institut Pasteur, 75015 Paris, France.
7
Laboratory of Biology & Genetics of the Bacterial Cell Wall, Department of Microbiology, Institut Pasteur, 75015 Paris, France; INSERM, Equipe Avenir, 75015 Paris, France.
8
Center for Human Immunology, Institut Pasteur, 75015 Paris, France; Laboratory of Immunoregulation, Department of Immunology, Institut Pasteur, 75015 Paris, France.
9
Myriad Rules Based Medicine, Inc., 72770 Reutlingen, Germany.
10
Laboratory of Human Evolutionary Genetics, Department of Genomes & Genetics, Institut Pasteur, 75015 Paris, France; CNRS URA3012, 75015 Paris, France. Electronic address: quintana@pasteur.fr.
11
Center for Human Immunology, Institut Pasteur, 75015 Paris, France; INSERM U818, 75015 Paris, France; Laboratory of Dendritic Cell Immunobiology, Department of Immunology, Institut Pasteur, 75015 Paris, France; INSERM UMS20, 75015 Paris, France. Electronic address: albertm@pasteur.fr.

Abstract

Standardization of immunophenotyping procedures has become a high priority. We have developed a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess induced innate or adaptive immune responses. By eliminating preanalytical errors associated with immune monitoring, we have defined the protein signatures induced by (1) medically relevant bacteria, fungi, and viruses; (2) agonists specific for defined host sensors; (3) clinically employed cytokines; and (4) activators of T cell immunity. Our results provide an initial assessment of healthy donor reference values for induced cytokines and chemokines and we report the failure to release interleukin-1α as a common immunological phenotype. The observed naturally occurring variation of the immune response may help to explain differential susceptibility to disease or response to therapeutic intervention. The implementation of a general solution for assessment of functional immune responses will help support harmonization of clinical studies and data sharing.

PMID:
24656047
DOI:
10.1016/j.immuni.2014.03.002
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center