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Ocul Immunol Inflamm. 2015 Jun;23(3):194-204. doi: 10.3109/09273948.2014.891754. Epub 2014 Mar 21.

In Vitro Immunomodulatory Effects of Human Corneal Stromal Cells on Human Th17 Cells.

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1
Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea; and Laboratory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute , Seoul , Korea and.

Abstract

PURPOSE:

To investigate the immunomodulatory effects of human corneal stromal cells on the function of Th17 cells, in vitro.

METHODS:

Th17 cells were co-cultured with corneal stromal cells with or without 1-methyltryptophan, 1400w dihydrochloride, interleukin (IL)-10 inhibitor, or transforming growth factor-beta (TGF-β) inhibitor. The secretion of IL-17A by Th17 cells and the upregulation of T regulatory (Treg) cell frequencies were evaluated. Transwell assays were conducted to evaluate whether the immunomodulatory effects of stromal cells were dependent on cell-to-cell contact.

RESULTS:

Corneal stromal cells suppressed the secretive function of Th17 cells and upregulated Treg cell frequencies, enhancing TGF-β. Tranwell assays showed similar immunomodulatory effects of the stromal cells on the function of Th17 cells. TGF-β inhibitors attenuated the suppressive effects of the stromal cells on the function of Th17 cells, whereas other inhibitors didn't.

CONCLUSION:

It which indicates that human corneal stromal cells suppress the function of Th17 cells through a TGF-β-dependent pathway.

KEYWORDS:

Cornea; IL-17; T cells; T regulatory cells; TGF-β; Th17 cells; stromal cells

PMID:
24654747
DOI:
10.3109/09273948.2014.891754
[Indexed for MEDLINE]
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