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Science. 2014 Mar 21;343(6177):1249531. doi: 10.1126/science.1249531.

Nucleocytoplasmic shuttling of a GATA transcription factor functions as a development timer.

Author information

1
Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.

Abstract

Biological oscillations are observed at many levels of cellular organization. In the social amoeba Dictyostelium discoideum, starvation-triggered multicellular development is organized by periodic cyclic adenosine 3',5'-monophosphate (cAMP) waves, which provide both chemoattractant gradients and developmental signals. We report that GtaC, a GATA transcription factor, exhibits rapid nucleocytoplasmic shuttling in response to cAMP waves. This behavior requires coordinated action of a nuclear localization signal and reversible G protein (heterotrimeric guanine nucleotide-binding protein)-coupled receptor-mediated phosphorylation. Although both are required for developmental gene expression, receptor occupancy promotes nuclear exit of GtaC, which leads to a transient burst of transcription at each cAMP cycle. We demonstrate that this biological circuit filters out high-frequency signals and counts those admitted, thereby enabling cells to modulate gene expression according to the dynamic pattern of the external stimuli.

PMID:
24653039
PMCID:
PMC4061987
DOI:
10.1126/science.1249531
[Indexed for MEDLINE]
Free PMC Article

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