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Food Chem Toxicol. 2014 Jun;68:71-7. doi: 10.1016/j.fct.2014.02.036. Epub 2014 Mar 17.

Evaluation of toxicity of essential oils palmarosa, citronella, lemongrass and vetiver in human lymphocytes.

Author information

1
Department of Botany, Centre of Advanced Study, Cell Biology and Genetic Toxicology Laboratory, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
2
Department of Botany, Centre of Advanced Study, Cell Biology and Genetic Toxicology Laboratory, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India. Electronic address: anitamukherjee28@gmail.com.

Abstract

The present investigation was undertaken to study the cytotoxic and genotoxic potential of the essential oils (palmarosa, citronella, lemongrass and vetiver) and monoterpenoids (citral and geraniol) in human lymphocytes. Trypan blue dye exclusion and MTT test was used to evaluate cytotoxicity. The genotoxicity studies were carried out by comet and DNA diffusion assays. Apoptosis was confirmed by Annexin/PI double staining. In addition, generation of reactive oxygen species was evaluated by DCFH-DA staining using flow cytometry. The results demonstrated that the four essential oils and citral induced cytotoxicity and genotoxicity at higher concentrations. The essential oils were found to induce oxidative stress evidenced by the generation of reactive oxygen species. With the exception of geraniol, induction of apoptosis was confirmed at higher concentrations of the test substances. Based on the results, the four essential oils are considered safe for human consumption at low concentrations.

KEYWORDS:

Apoptosis; Cytotoxicity; Essential oils; Genotoxicity; Human lymphocytes; Reactive oxygen species

PMID:
24650756
DOI:
10.1016/j.fct.2014.02.036
[Indexed for MEDLINE]

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