Prognostic value of type XXII and XXIV collagen mRNA expression in head and neck cancer patients

Kiyoshi Misawa; Takeharu Kanazawa; Atsushi Imai; Shiori Endo; Daiki Mochizuki; Hirofumi Fukushima; Yuki Misawa; Hiroyuki Mineta

doi:10.3892/mco.2013.233

Prognostic value of type XXII and XXIV collagen mRNA expression in head and neck cancer patients

Mol Clin Oncol. 2014 Mar;2(2):285-291. doi: 10.3892/mco.2013.233. Epub 2013 Dec 24.

Authors

Kiyoshi Misawa¹, Takeharu Kanazawa², Atsushi Imai¹, Shiori Endo¹, Daiki Mochizuki¹, Hirofumi Fukushima³, Yuki Misawa¹, Hiroyuki Mineta¹

Affiliations

¹ Department of Otolaryngology/Head and Neck Surgery, Hamamatsu University School of Medicine, Shizuoka, Japan.
² Department of Otolaryngology/Head and Neck Surgery, Jichi Medical University, Tochigi, Japan.
³ Department of Head and Neck, Cancer Institute Hospital of Japanese Foundation for Cancer Research, Tokyo, Japan.

Abstract

Squamous cell carcinoma of the head and neck (HNSCC) is the sixth most common type of cancer, affecting ~500,000 individuals worldwide annually. Collagen is the major constituent of the extracellular matrix component in tumors and plays a crucial role in tumor development. The aim of this study was to determine the mRNA expression of type XXI, XXII, XXIII and XXIV α1 collagen (COL21A1, COL22A1, COL23A1 and COL24A1, respectively) in head and neck squamous cell carcinoma (HNSCC) and investigate its correlation with disease progression. This study investigated the mRNA expression levels of COL21A1, COL22A1, COL23A1 and COL24A1 in 70 HNSCC primary samples and 44 matched pairs of tumor and adjacent normal mucosal tissues using quantitative polymerase chain reaction (qPCR). Expression data were compared to the clinicopathological variables in order to determine the correlation between expression and disease progression. Our results demonstrated that the mRNA levels of COL22A1 and COL24A1 were significantly higher in HNSCC tissues compared to those in the corresponding normal tissues from the same individuals (n=44; P<0.001 and P=0.019, respectively). The COL22A1 mRNA levels were found to be significantly associated with lymph node metastasis (P=0.018) and pathological stage (P=0.024), whereas the COL24A1 mRNA levels were significantly associated with tumor size (P=0.045). The high expression levels of COL22A1 and COL24A1 mRNA were statistically correlated with a decrease in disease-free survival (DFS) (log-rank test, P<0.001). The results of the multivariate logistic regression analysis revealed that high expression levels of the COL22A1 and COL24A1 gene pair were associated with a high odds ratio for recurrence of 14.62 (95% confidence interval: 2.77-77.26; P=0.002). Therefore, the upregulation of COL22A1 and COL24A1 mRNA may play a critical role in the progression of HNSCC and provide useful information as a prognostic predictor for HNSCC patients.

Keywords: head and neck squamous cell carcinoma; mRNA expression; quantitative polymerase chain reaction; type XXII α1 collagen; type XXIV α1 collagen.