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Eur J Immunol. 2014 Jun;44(6):1615-21. doi: 10.1002/eji.201343822. Epub 2014 Mar 11.

Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts.

Author information

1
Allergie-Centrum-Charité, CCM, Klinik für Dermatologie, Venerologie und Allergologie, Charité-Universitäts-medizin Berlin, Berlin, Germany; Cell Biology Group, Deutsches Rheuma-Forschungszentrum Berlin (DRFZ), Berlin, Germany.

Abstract

B-cell-derived interleukin-10 (IL-10) is known to act in a paracrine fashion to suppress inflammation. Here, we show that IL-10 also acts in an autocrine manner to regulate the differentiation of activated human B cells. We report that IL-10 expression is not restricted to a dedicated B-cell subset, but is induced transiently in peripheral human naïve, memory, and CD5(+) B cells alike upon activation. Global transcriptome comparison of activated human B cells, secreting IL-10 or not, identified 138 differentially regulated genes, most of which were associated with differentiation into antibody-secreting cells and reflecting autocrine IL-10 signaling. We monitored the differentiation of IL-10-secreting B cells and determined the effect of IL-10-blocking antibodies against its autocrine and paracrine signaling. IL-10 signaling promoted the differentiation of activated IL-10-secreting B cells into IgM- or IgG-secreting cells, but was dispensable for IgA secretion. Our data imply that B-cell-derived IL-10 not only suppresses immune reactions via paracrine mechanisms, but can also contribute to the differentiation of IL-10-secreting B cells into IgM- and IgG-secreting plasmablasts through both autocrine and paracrine signaling.

KEYWORDS:

Antibody-secreting cell; Human B cells; IL-10; Transcriptome

PMID:
24643722
DOI:
10.1002/eji.201343822
[Indexed for MEDLINE]
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