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Biochem Biophys Res Commun. 2014 Apr 18;446(4):894-900. doi: 10.1016/j.bbrc.2014.03.027. Epub 2014 Mar 15.

Downregulation of adenomatous polyposis coli by microRNA-663 promotes odontogenic differentiation through activation of Wnt/beta-catenin signaling.

Author information

1
Oral Biology Research Institute, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea.
2
Department of Biomedical Science, Chosun University, Gwangju 501-759, Republic of Korea.
3
Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, BK 21, Seoul National University, Seoul 110-749, Republic of Korea.
4
Oral Biology Research Institute, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea. Electronic address: kdk@chosun.ac.kr.

Abstract

MicroRNAs (miRNAs) regulate cell differentiation by inhibiting mRNA translation or by inducing its degradation. However, the role of miRNAs in odontogenic differentiation is largely unknown. In this present study, we observed that the expression of miR-663 increased significantly during differentiation of MDPC-23 cells to odontoblasts. Furthermore, up-regulation of miR-663 expression promoted odontogenic differentiation and accelerated mineralization without proliferation in MDPC-23 cells. In addition, target gene prediction for miR-663 revealed that the mRNA of the adenomatous polyposis coli (APC) gene, which is associated with the Wnt/β-catenin signaling pathway, has a miR-663 binding site in its 3'-untranslated region (3'UTR). Furthermore, APC expressional was suppressed significantly by miR-663, and this down-regulation of APC expression triggered activation of Wnt/β-catenin signaling through accumulation of β-catenin in the nucleus. Taken together, these findings suggest that miR-663 promotes differentiation of MDPC-23 cells to odontoblasts by targeting APC-mediated activation of Wnt/β-catenin signaling. Therefore, miR-663 can be considered a critical regulator of odontoblast differentiation and can be utilized for developing miRNA-based therapeutic agents.

KEYWORDS:

Adenomatous polyposis coli; Differentiation; Odontoblasts; miR-663; β-Catenin

PMID:
24642258
DOI:
10.1016/j.bbrc.2014.03.027
[Indexed for MEDLINE]
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