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Biochem Biophys Res Commun. 2014 Apr 18;446(4):959-64. doi: 10.1016/j.bbrc.2014.03.033. Epub 2014 Mar 15.

The formation of brown adipose tissue induced by transgenic over-expression of PPARγ2.

Author information

1
Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Sciences, Huazhong Agricultural University, Wuhan 430070, China.
2
Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Sciences, Huazhong Agricultural University, Wuhan 430070, China; Dept of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, HI, USA. Electronic address: jinzeng@hawaii.edu.
3
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
4
Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Sciences, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: renzq@mail.hzau.edu.cn.
5
Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Sciences, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: xiongyzy@163.com.

Abstract

Brown adipose tissue (BAT) is specialized to dissipate energy as heat, therefore reducing fat deposition and counteracting obesity. Brown adipocytes arise from myoblastic progenitors during embryonic development by the action of transcription regulator PRDM16 binding to PPARγ, which promotes BAT-like phenotype in white adipose tissue. To investigate the capability of converting white adipose tissue to BAT or browning by PPARγ in vivo, we generated transgenic mice with over-expressed PPARγ2. The transgenic mice showed strong brown fat features in subcutaneous fat in morphology and histology. To provide molecular evidences on browning characteristics of the adipose tissue, we employed quantitative real-time PCR to determine BAT-specific gene expressions. The transgenic mice had remarkably elevated mRNA level of UCP1, Elovl3, PGC1α and Cebpα in subcutaneous fat. Compared with wild-type mice, UCP1 protein levels were increased significantly in transgenic mice. ATP concentration was slightly decreased in the subcutaneous fat of transgenic mice. Western blotting analysis also confirmed that phosphorylated AMPK and ACC proteins were significantly (P<0.01) increased in the transgenic mice. Therefore, this study demonstrated that over-expression of PPARγ2 in skeletal muscle can promote conversion of subcutaneous fat to brown fat formation, which can have beneficial effects on increasing energy metabolisms and combating obesity.

KEYWORDS:

Brown adipose tissue; Over-expression; PPARγ2; Transgenic mice; UCP1; White adipose tissue

PMID:
24642257
DOI:
10.1016/j.bbrc.2014.03.033
[Indexed for MEDLINE]

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